Final answer:
In replicative transposition, a copy of the insertion sequence is made by local DNA replication and pasted at a new insertion site in the genome. This process involves the transposase binding to the transposon ends, catalyzing a hydrolytic attack of DNA at the new insertion site, and then priming replication of the transposon strands from the 3'OH ends of the insertion site DNA strands. The result is a cointegrate structure that is resolved by recombination, leaving copies of the transposon at both the original site and the new insertion site.
Step-by-step explanation:
In replicative transposition, a copy of the insertion sequence is made by local DNA replication and pasted at a new insertion site in the genome. This process involves the transposase binding to the transposon ends, catalyzing a hydrolytic attack of DNA at the new insertion site, and then priming replication of the transposon strands from the 3'OH ends of the insertion site DNA strands.
The result is a cointegrate structure that is resolved by recombination, leaving copies of the transposon at both the original site and the new insertion site.