Final answer:
At 230 nm, 260 nm, and 280 nm, you would measure the absorbance of certain compounds or substances.
Step-by-step explanation:
At 230 nm, you would measure the absorbance of certain compounds or substances. At 260 nm, you would also measure the absorbance of certain compounds or substances. And at 280 nm, you would measure the absorbance of certain compounds or substances. These measurements are often used in spectroscopy to determine the concentration or purity of a substance. Measurements at specific wavelengths, such as 230 nm, 260 nm, and 280 nm, are used to determine the concentration and purity of nucleic acids and proteins in solution. At 260 nm, the absorbance value is often used to estimate the concentration of nucleic acids, such as DNA and RNA, because nucleic acids absorb ultraviolet light maximally at this wavelength. At 280 nm, the absorbance is indicative of the presence of aromatic amino acids, such as tryptophan and tyrosine, which are components of proteins; proteins often have a peak absorbance around this wavelength. In contrast, 230 nm measurements can indicate contamination or be used for other specific analytical purposes.