Question

For average organism, light digestion of chromatin with micrococcal nuclease would yield a ladder on a gel with bands every ________ base pairs.

-200
-1,000
-352
-146

Answer 1

For the average organism, light digestion of chromatin with micrococcal nuclease would yield a ladder on a gel with bands every 200 base pairs. Option A is the answer.

Step-by-step explanation:

Micrococcal nuclease is an enzyme that cleaves DNA at the linker regions between nucleosomes, which are repeating units of chromatin. Chromatin is the complex of DNA and proteins in the nucleus of a cell. When chromatin is subjected to light digestion with micrococcal nuclease, it results in the formation of DNA fragments corresponding to the nucleosomal repeat. For the average organism, these fragments typically have a length of around 200 base pairs, reflecting the characteristic spacing between nucleosomes. This pattern, visualized as a ladder on a gel, demonstrates the regular packing of DNA around nucleosomes.

Option A is the answer.