Final answer:
RNA polymerases I and II have promoters that are located upstream of the startpoint of transcription. RNA polymerase III has some genes it transcribes with internal promoters within the transcribed sequence.
Step-by-step explanation:
The question addresses which eukaryotic RNA polymerases have promoters located upstream of the startpoint of transcription. In eukaryotes, RNA polymerase I, RNA polymerase II, and RNA polymerase III are responsible for the synthesis of different types of RNA. RNA polymerase I and RNA polymerase II have promoters that are located upstream. RNA polymerase I transcribes genes with two GC-rich promoter sequences in the -45 to +20 region and can be further enhanced by sequences from -180 to -105 upstream of the initiation site. On the other hand, RNA polymerase II transcribes protein-coding genes with a complex initiation process involving several transcription factors binding to promoter sequences upstream of genes, typically featuring a TATA box around -25 to -35 bases relative to the initiation site.
However, RNA polymerase III is unique in that some of the genes it transcribes, such as 5S rRNA and tRNAs, have internal promoters within the transcribed sequence, rather than upstream. Hence, the correct answer to the question is 'RNA polymerases I and II'. In summary, RNA polymerases I and II have at least some promoters located upstream of the startpoint of transcription.