Question

Which of the following have been used to affinity purify Mediator from nuclear extracts?

-Sin4 and Gal 11
-SCAF and pTEFb
-TFIIB N-terminal peptides and TAN-1
-VP16 and synthetic CTD peptides

Answer 1

Sin4 and Gal11 are components used for affinity purification of the Mediator complex from nuclear extracts, as they are Mediator subunits that facilitate the purification process.

Step-by-step explanation:

The Mediator complex has been affinity purified from nuclear extracts using various methods. Among the list provided, Sin4 and Gal11 are known to be components used for this purpose. Sin4 and Gal11 are mediator complex proteins and can serve as baits due to their interactions with other Mediator subunits, thereby enabling the purification of the entire complex from the nuclear extracts.

Conversely, options such as SCAF and pTEFb, TFIIB N-terminal peptides and TAN-1, and VP16 and synthetic CTD peptides don't align with the known methodologies for Mediator purification, as they don't target Mediator subunits for this specific process.