Question

Assembly of the PIC: Which of the following statements is FALSE?

-TFIIE binds after TFIIF and before TFIIH.
-The first basal factor to be recruited to the preinitiation complex is TFIID (or at some promoters SAGA).
-When TBP operates at an RNA pol II promoter, it is often part of a complex of other proteins called TAFs.
-RNA pol II binds the promoter first and then recruits TFIID and then either TFIIA or TFIIB.

Answer 1

The false statement is that TFIIE binds after TFIIF and before TFIIH. The correct assembly of the PIC involves TFIID, TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II.

Step-by-step explanation:

The FALSE statement is: TFIIE binds after TFIIF and before TFIIH. The correct order of assembly of the PIC (preinitiation complex) is as follows:

1. TFIID (first basal factor) binds to the TATA box.
2. TFIIA and TFIIB are recruited next.
3. TFIIE, TFIIF, and TFIIH are then assembled, along with other initiation factors.
4. RNA polymerase II binds the promoter last.

This sequence of events ensures the proper formation of the transcription initiation complex and allows RNA polymerase II to start transcribing the mRNA.