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Which of the following statements is confirmed by the Western blot shown?

A. SWR1 deposits H2AZ histones into the nucleosome arrays.
B. SWR1 function is not ATP-dependent.
C. The antibody used in this experiment binds to the SWR1 complex.
D. All of the above.

User Shuo
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1 Answer

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Final answer:

The Western blot can confirm that SWR1 deposits H2AZ histones into the nucleosome arrays. The other statements regarding ATP dependency and antibody binding to the SWR1 complex cannot be directly confirmed by a Western blot without additional context.

Step-by-step explanation:

Among the options provided, the statement that can be confirmed by the Western blot is that SWR1 deposits H2AZ histones into the nucleosome arrays. A Western blot typically detects proteins, including histones, using specific antibodies. If the antibody used is specific to the SWR1 complex, it would detect its presence by identifying proteins associated with it, specifically H2AZ histones being part of the nucleosome arrays due to the activity of SWR1. The fact that SWR1 deposits H2AZ histones is related to its role in chromatin remodeling, which can be investigated through chromatin extraction techniques that reveal changes to nucleosome positioning. ATP dependency is not directly assessed by a Western blot; it is usually tested by biochemical experiments looking for the effect of ATP on SWR1 activity. The binding of the antibody to the SWR1 complex itself would not be confirmed by a Western blot that's aimed at detecting histones unless an antibody against the SWR1 complex was used for detection, which is not specified in the given options.

User Tarleb
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