Question

Steps in RecA production. What distinguishes the binding sites of RecA?

Answer 1

The steps in RecA production involve 5'-3' exonuclease activity, RecA protein binding to single-stranded DNA, and the formation of a nucleoprotein filament. What distinguishes the binding sites of RecA is that RecA protein monomers have multiple DNA binding sites.

Step-by-step explanation:

The steps in RecA production are as follows:

1. A 5'-3' exonuclease trims template DNA back along its complement.
2. RecA protein monomers bind to the single-stranded DNA to form a nucleoprotein filament.
3. The filament scans the 'other' replicating strand for homologous sequences.
4. When homologous sequences are found, the RecA-DNA filament binds to them.
5. The filament of new DNA 'invades' the homologous double-stranded DNA.
6. Leading strand replication continues from the 3' end of the invading strand
7. A new replication fork is established, and lagging strand replication resumes at the new fork.

What distinguishes the binding sites of RecA is that RecA protein monomers have multiple DNA binding sites, allowing them to bind to single-stranded DNA and form a nucleoprotein filament.