Final answer:
During metaphase of cell division, chromosomes align along the midline of the cell.
Step-by-step explanation:
The correct answer is E) metaphase.
During metaphase of cell division, the chromosomes align along the midline or equator of the cell. This alignment is important for the equal distribution of chromosomes to the resulting daughter cells. The chromosomes are maximally condensed during metaphase and are attached to spindle fibers from opposing poles of the cell. Once the chromosomes are properly aligned at the metaphase plate, they can be separated and pulled to opposite poles of the cell during anaphase.