Final answer:
Increasing substrate concentration in an enzyme-catalyzed reaction, while keeping the enzyme concentration constant, will not reach Vmax due to substrate saturation at all enzyme active sites.
Step-by-step explanation:
Increasing the substrate concentration in an enzyme-catalyzed reaction, while keeping the enzyme concentration constant, will not reach Vmax. This is because at some point, all of the enzyme's active sites become occupied by substrate molecules, and the reaction rate levels off. Further increases in substrate concentration will not increase the rate of the reaction. This is known as substrate saturation.