Question

Each subunit of Tn5-Tn10 dimer has an active site that creates ___________ at the end of the transposon and then creates a _________ at the target site

Answer 1

Each subunit of the Tn5-Tn10 dimer has an active site that creates staggered ends at the transposon end and staggered cuts at the target site, mediated by the enzyme transposase.

Step-by-step explanation:

Each subunit of Tn5-Tn10 dimer has an active site that creates staggered ends at the end of the transposon and then creates a staggered cut at the target site. The enzyme responsible for this action is transposase, which catalyzes the excision and integration of the transposon DNA during the process known as transposition. After creating staggered ends in the transposon, the transposase brings the ends together to mediate insertion into a new site in the DNA. Insertion begins with 3'-to-5' ligation of one strand at one end of the transposon to the target DNA. Following the ligation, replication fills in the missing bases on the transposon, resulting in direct repeats of host cell genomic DNA at the insertion site, and a final ligation step completes the transposition.