Question

Which techniques can be used to identify what proteins are being expressed in a cell? (Check all that apply.)

a) Western blotting
b) Polymerase chain reaction (PCR)
c) Mass spectrometry
d) Enzyme-linked immunosorbent assay (ELISA)

Answer 1

Techniques for identifying proteins in a cell include Western blotting, mass spectrometry, and ELISA.

Step-by-step explanation:

The techniques that can be used to identify what proteins are being expressed in a cell include:

1. Western blotting: This technique uses antibodies to detect specific proteins in a gel by transferring them to a nitrocellulose membrane and adding a secondary antibody coupled to an enzyme or fluorophore.
2. Mass spectrometry: This technology is used to identify and analyze the characteristics of proteins by measuring their mass-to-charge ratio.
3. Enzyme-linked immunosorbent assay (ELISA): ELISA utilizes antibodies to detect the presence of specific antigens and can be conducted in microtiter plates or in vivo.