Question

Genomic libraries are often screened using probes derived from organisms that are different than the one from which the library was generated. Therefore, there are often mismatches between the probe and the most similar matching clones in the library. As a result, it's possible that no matching clones may initially be identified by hybridization.

Which of the following methods would likely increase the chances of identifying the most similar matching clones?"

A. Using a shorter probe.
B. Using a longer probe.
C. Lowering the stringency of hybridization conditions.
D. Using a probe from a more distantly related organism.

Answer 1

To increase the chances of identifying the most similar matching clones in a genomic library screening, using a longer probe, lowering the stringency of hybridization conditions, and using a probe from a more distantly related organism can be effective.

Step-by-step explanation:

When screening a genomic library using probes derived from different organisms, there may be mismatches between the probe and the clones in the library. To increase the chances of identifying the most similar matching clones, there are several methods that can be used:

1. Using a longer probe: A longer probe increases the likelihood of finding a closer match in the library.
2. Lowering the stringency of hybridization conditions: By reducing the stringency, mismatches between the probe and the clones are tolerated, increasing the chances of identifying matching clones.
3. Using a probe from a more distantly related organism: A probe from a more distantly related organism may have fewer mismatches with the clones in the library, increasing the chances of finding a match.