Final answer:
A restriction fragment is generated by the action of a restriction enzyme, also known as an endonuclease.
Step-by-step explanation:
A restriction fragment is generated by the action of a restriction enzyme, also known as an endonuclease. This statement is true.
Restriction endonucleases are enzymes that recognize specific DNA sequences and cleave the DNA at those sites. When a restriction enzyme acts on a large DNA molecule, it breaks it down into many smaller fragments of different sizes. These fragments can be visualized on a gel and used in various applications, such as DNA fingerprinting and recombinant DNA technology.