Question

Suppose that two proteins, A and B, can associate with each other to form a complex. You measure the dissociation constant at several different temperatures and find that as you heat up the sample, the Kd decreases. Does this mean that binding of A and B becomes more favorable or less favorable as the temperature increases?

Answer 1

The binding of proteins A and B becomes more favorable as the temperature increases.

Step-by-step explanation:

As you heat up the sample, the dissociation constant (Kd) decreases. This means that the binding of proteins A and B becomes more favorable as the temperature increases. The relationship between Kd and temperature is influenced by the enthalpy change (ΔH°) of the reaction.

In an exothermic reaction (ΔH° < 0), the magnitude of Kd decreases with increasing temperature, while in an endothermic reaction (ΔH° > 0), the magnitude of Kd increases with increasing temperature.