Question

12) You have the amino acid sequence of a protein and wish to search for the gene encoding this protein in a DNA library. Using this protein sequence, you deduce a particular DNA sequence that can encode this protein. Why is it unwise to use only this DNA sequence you have deduced as the probe for isolating the gene encoding your protein of interest from the DNA library?

Answer 1

Using only the deduced DNA sequence is unwise because the genetic code is degenerate and there are multiple possible coding sequences for a given amino acid sequence.

Step-by-step explanation:

It is unwise to use only the deduced DNA sequence as the probe for isolating the gene encoding the protein in a DNA library because the genetic code is degenerate.

his means that multiple codons can encode the same amino acid. Therefore, there are multiple possible coding sequences for a given amino acid sequence. Using only the deduced DNA sequence would eliminate the possibility of identifying other potential coding sequences for the protein of interest.