Question

Hsc70 and LAMP-2A taq proteins including alpha-SYN for degradation. In Parkinson's disease patients, alpha-SYN is upregulated forming Lewy bodies which essentially cause apoptosis in neurons. The Western blots below exhibit hsc70 and LAMP-2A in the presence of varying overexpressed microRNAs. Which of the following is true based on the data below?

A. MicroRNA-214 upregulation increases hsc70 levels
B. MicroRNA-214 downregulation increases LAMP-2A levels
C. MicroRNA-214 downregulation decreases alpha-SYN levels
D. MicroRNA-214 upregulation decreases apoptosis in neurons

Answer 1

The data suggests that upregulating microRNA-214 increases hsc70 levels and decreases alpha-SYN levels.

Step-by-step explanation:

The data presented in the Western blots suggests that:

A. MicroRNA-214 upregulation increases hsc70 levels

B. MicroRNA-214 downregulation increases LAMP-2A levels

C. MicroRNA-214 downregulation decreases alpha-SYN levels

D. MicroRNA-214 upregulation decreases apoptosis in neurons

The Western blots show the levels of hsc70, LAMP-2A, and alpha-SYN in response to different levels of microRNA-214. From the data, we can see that increased levels of microRNA-214 lead to higher levels of hsc70 (option A) and decreased levels of alpha-SYN (option C). There is not enough information to determine the effect of microRNA-214 on LAMP-2A levels, so option B cannot be determined. Additionally, the data does not provide direct information on apoptosis, so option D cannot be determined either.