Question

Working in a world-class proteomics lab you purify a new peptide with the amino acid sequence reading GFGILAVI. BAsed on the nature of these amino acids you believe that the sequence is part of:

a)a protein destined for the peroxisome
b)a protein destined for the nucleus
c)a protein destined for the chloroplast
d)a protein that is most likely attached to the cytosolic side of a transmembrane
e)a protein that is most likely a single pass transmembrane protein in the ER

Answer 1

Considering the hydrophobic nature of the amino acid sequence GFGILAVI, the peptide is likely part of a membrane-associated protein, potentially as a transmembrane domain.

Step-by-step explanation:

Based on the amino acid sequence GFGILAVI and the principles of protein targeting and signal sequences, it is logical to deduce the likely cellular destination of the protein. The specified sequence is made up mostly of hydrophobic amino acids, suggesting that it could be a part of a protein that interacts with a membrane. Given this information, the sequence could be involved in the protein being anchored in a membrane or spanning a membrane.

Considering options a to e, the most likely role based on intrinsic hydrophobicity would be (d) a protein that is most likely attached to the cytosolic side of a transmembrane or (e) a protein that is most likely a single pass transmembrane protein in the ER with an internal hydrophobic stop-transfer sequence trapping the protein within the membrane.