Question

Which technique can be used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

a) DNA microarray
b) Treatment with reverse transcriptase to isolate the DNA of interest
c) BLAST analysis
d) Mass spectrometry

Answer 1

Mass spectrometry is the technique used for identifying proteins after two-dimensional gel electrophoresis, providing detailed protein analysis.

Step-by-step explanation:

After conducting a two-dimensional gel electrophoresis experiment to separate proteins, the technique that can be used to identify a protein of interest is mass spectrometry. This approach is critical for protein analysis and is capable of identifying the molecular characteristics of the proteins separated by the electrophoresis. Mass spectrometry is a highly sensitive technique that can analyze very small samples of protein and provide information about its mass and sequence for accurate identification.