Final answer:
The ΔG of ATP hydrolysis in the cell is approximately -12 kcal/mol, whereas the ΔG0' is 7.3 kcal/mol. The difference in ΔG values arises due to the difference in concentrations of ATP and its hydrolysis products (ADP and inorganic phosphate) between intracellular and standard conditions.
Step-by-step explanation:
The ΔG of ATP hydrolysis in the cell is approximately -12 kcal/mol, whereas the ΔG0' is 7.3 kcal/mol. The difference in ΔG values arises due to the difference in concentrations of ATP and its hydrolysis products (ADP and inorganic phosphate) between intracellular and standard conditions.
Under cellular conditions, the concentration of ATP is much higher than that of ADP and inorganic phosphate, resulting in a higher free energy release during ATP hydrolysis. This increased concentration ratio drives the reaction towards the formation of ADP and inorganic phosphate, releasing more free energy.
The concentration ratio can be calculated using the formula ΔG = ΔG0' + RT ln([ADP][P]/[ATP]), where R is the gas constant and T is the temperature in Kelvin.