Question

What does detection of a fluorescent signal from one of the spots of a DNA microarray indicate?

a) The probe hybridized to the sequences present in that spot
b) The probe did not hybridize to that particular gene
c) The experiment was not conducted correctly
d) That the probe was non-specifically trapped by the material present in the spot

Answer 1

The detection of a fluorescent signal from a spot on a DNA microarray indicates that the probe hybridized to the sequences present in that spot.

Step-by-step explanation:

The detection of a fluorescent signal from one of the spots of a DNA microarray indicates that the probe hybridized to the sequences present in that spot.

When the probe, which is a short piece of DNA complementary to the sequence of interest, binds to the target DNA fragments in the spot, it generates a fluorescent signal that can be visualized.