Final answer:
The major crisis for Puritans in Massachusetts in the seventeenth century was the annulment of Massachusetts' charter.
Step-by-step explanation:
The major crisis for Puritans in Massachusetts in the seventeenth century was the annulment of Massachusetts' charter. The charter was revoked in 1684 by King James II, who wanted to increase royal control over the colonies. This event led to the loss of self-government and religious freedoms for the Puritans in Massachusetts.