Final answer:
The osmolarity in the ascending limb of the Loop of Henle is hypo-osmotic compared to the interstitial fluid because of the reabsorption of water in the descending limb.
Step-by-step explanation:
The osmolarity in the ascending limb of the Loop of Henle is hypO-osmotic compared to the interstitial fluid immediately surrounding it.
This is because of the reabsorption of water that occurs in the descending limb of the loop.
As water moves out of the descending limb and into the interstitial fluid, the concentration of solutes in the filtrate increases, making it hypO-osmotic.
Therefore, option 1) 'It's hypo-osmotic because of the reabsorption of water' is the correct answer.