Question

You incubate eukaryotic cells for 30 minutes in H -uridine and then chase them in unlabeled media for about an hour before the cells are killed and the RNA is extracted. Where does the radiolabel appear after this experiment?

1) in larger RNAs in the cytoplasm
2) in smaller RNAs in the mitochondria
3) in smaller RNAs in the cytoplasm
4) in larger RNAs in the mitochondria

Answer 1

The radiolabel would appear in larger RNAs in the cytoplasm.

Step-by-step explanation:

After incubating eukaryotic cells with H-uridine for 30 minutes and then chasing them in unlabeled media for about an hour, the radiolabel would appear in larger RNAs in the cytoplasm. This is because the cells were incubated with H-uridine, which is incorporated into newly synthesized RNA molecules. During the chase period, the newly synthesized RNA molecules are transported to the cytoplasm, where they can be detected.