Final answer:
The statement is false; during gene transcription, only one strand of DNA is used as a template to synthesize mRNA.
Step-by-step explanation:
The statement in the question is False.
During transcription of a gene, only one of the two nucleotide strands of DNA is used as a template to synthesize mRNA.
The template strand is complementary to the RNA product, while the other DNA strand, called the nontemplate strand, is almost identical to the RNA transcript but with T nucleotides replaced by U nucleotides.