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What must be preassembled on the promoter before transcription can begin?

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Final answer:

Before transcription can begin, the TATA box and transcription factors need to be preassembled on the promoter.

Step-by-step explanation:

Before transcription can begin, the promoter needs to have several components preassembled. The promoter is the DNA sequence to which RNA polymerase and associated factors bind to initiate transcription. One important component that must be preassembled on the promoter is the TATA box, which is a repeat of thymine and adenine dinucleotides. Transcription factors, such as TFIID, TFIIB, and others, also need to bind to the TATA box and recruit RNA polymerase to the promoter.

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