Question

Gene cloning requires placing the gene of interest into a cloning vector. A good cloning vector must have which of the following?

1) Ability to self-replicate
2) Multiple restriction enzyme recognition sites
3) Antibiotic resistance gene
4) All of the above

Answer 1

A good cloning vector must have the ability to self-replicate, multiple restriction enzyme recognition sites, and an antibiotic resistance gene; therefore, the correct answer is (4) All of the above.

Step-by-step explanation:

A good cloning vector must have several important features to function effectively in gene cloning. Firstly, it must have the ability to self-replicate, ensuring that once it's introduced into a host cell, it can multiply independently of the host's chromosomal DNA. Secondly, the presence of multiple restriction enzyme recognition sites, often in the form of a multiple cloning site (MCS), is crucial for the easy insertion of foreign DNA fragments into the vector. Lastly, an antibiotic resistance gene is generally included as a selectable marker, which helps in identifying and maintaining the cells that have been successfully transformed with the recombinant vector.

To address the question, the correct answer would be (4) All of the above, as a good cloning vector requires all these mentioned elements to facilitate the cloning process effectively.