Question

Which of the following microscopic techniques supports three dimensional reconstruction from optical sections obtained from a fluorescent specimen?

A) Transmission Electron Microscopy (TEM)
B) Scanning Electron Microscopy (SEM)
C) Confocal Laser Scanning Microscopy (CLSM)
D) Phase-Contrast Microscopy (PCM)

Answer 1

Confocal Laser Scanning Microscopy (CLSM) supports three-dimensional reconstruction from optical sections obtained from a fluorescent specimen.

Step-by-step explanation:

Confocal Laser Scanning Microscopy (CLSM) is a microscopic technique that supports three-dimensional reconstruction from optical sections obtained from a fluorescent specimen. It uses a laser to scan a sample point by point, collecting fluorescence emission from each point. This information is then used to reconstruct a 3D image of the specimen.

Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and Phase-Contrast Microscopy (PCM) do not support three-dimensional reconstruction from optical sections of a fluorescent specimen.