Question

After an automated dye primer sequencing run, the electropherogram displays consecutive peaks of the following colors: red, red, black, green, green, blue, black, red, green, black, blue, blue, blue. If the computer software displays the fluors from ddATP as green, ddCTP as blue, ddGTP as black, and ddTTP as red, what is the sequence of the region given?

Answer 1

The sequence of the region based on the given electropherogram is ddATP, ddATP, ddCTP, ddGTP, ddGTP, ddCTP, ddGTP, ddATP, ddGTP, ddCTP, ddCTP, ddCTP.

Step-by-step explanation:

The sequence of the region given can be determined by interpreting the consecutive peaks of colors in the electropherogram. According to the information provided, the fluors from ddATP are represented by red, ddCTP by black, ddGTP by green, and ddTTP by blue. Based on this, the sequence can be determined as follows:

1. Red, Red: ddATP, ddATP
2. Black: ddCTP
3. Green, Green: ddGTP, ddGTP
4. Blue: ddCTP
5. Black: ddGTP
6. Red: ddATP
7. Green: ddGTP
8. Black: ddCTP
9. Blue, Blue, Blue: ddCTP, ddCTP, ddCTP

Putting it all together, the sequence of the region is: ddATP, ddATP, ddCTP, ddGTP, ddGTP, ddCTP, ddGTP, ddATP, ddGTP, ddCTP, ddCTP, ddCTP.