Question

Using which of the following is an appropriate way to avoid PCR contamination?

1) Using separate work areas for DNA extraction and PCR setup
2) Wearing gloves and lab coat during PCR setup
3) Using filter tips for pipetting reagents
4) Performing PCR in a laminar flow hood

Answer 1

The appropriate way to avoid PCR contamination is by using separate work areas for DNA extraction and PCR setup, wearing gloves and a lab coat during PCR setup, using filter tips for pipetting reagents, and performing PCR in a laminar flow hood. Hence, all are true.

Step-by-step explanation:

The appropriate way to avoid PCR contamination is by using separate work areas for DNA extraction and PCR setup, wearing gloves and a lab coat during PCR setup, using filter tips for pipetting reagents, and performing PCR in a laminar flow hood.

By using separate work areas, you minimize the risk of cross-contamination between samples. Wearing gloves and a lab coat helps to minimize potential contamination from the user. Using filter tips reduces the risk of contamination from previous samples or reagents.

Performing PCR in a laminar flow hood provides a controlled and sterile environment, further reducing the chances of contamination.