Question

A northern blot is performed on an RNA transcript with the sequence GUAGGUATGUAUUUGGGCGCGAACGCAAAA. The probe sequence is GUAGGUATGUAUUUGGGCGCG. Will this probe hybridize to the target transcript?

1) Yes
2) No

Answer 1

The probe will not hybridize to the target transcript.

Step-by-step explanation:

In a Northern blot, RNA is transferred to a nylon membrane and probed. The probe sequence is GUAGGUATGUAUUUGGGCGCG. To determine if the probe will hybridize to the target transcript, we need to check if the probe sequence is complementary to the target transcript sequence. To do this, we can compare the two sequences: GUAGGUATGUAUUUGGGCGCGAACGCAAAA (target transcript) and GUAGGUATGUAUUUGGGCGCG (probe).

By comparing the two sequences, we can see that they have partial complementarity but are not completely complementary. Therefore, the probe will not hybridize to the target transcript.

Answer: 2) No