Question

A master mix of all components (except template) necessary for PCR contains what basic ingredients?

1) Primers, dNTPs, DNA polymerase, buffer, and water
2) Primers, RNA, DNA polymerase, buffer, and water
3) Primers, dNTPs, RNA polymerase, buffer, and water
4) Primers, dNTPs, DNA ligase, buffer, and water

Answer 1

A master mix of all components (except template) necessary for PCR includes primers, dNTPs, DNA polymerase, buffer, and water.

Step-by-step explanation:

A master mix of all components (except template) necessary for PCR contains the following basic ingredients:

1. Primers: short DNA sequences that bind to the target DNA
2. dNTPs: deoxynucleotide triphosphates that provide the building blocks for DNA synthesis
3. DNA polymerase: an enzyme that catalyzes the synthesis of new DNA strands
4. Buffer: a solution that maintains optimal pH and ionic conditions for the PCR reaction
5. Water: acts as a solvent and fills up the remaining volume in the reaction mixture