Final answer:
The technique that is not next-generation sequencing is tiled microarray, as it is used for gene expression analysis or genotyping rather than sequencing DNA.
Step-by-step explanation:
Identification of Non-Next-Generation Sequencing Technique
The student has asked which of the following is not a next-generation sequencing technique: 1) 454 sequencing, 2) tiled microarray, 3) SOLID system, 4) Illumina Genome Analyzer. Out of the given options, the tiled microarray is not a next-generation sequencing technology.
Next-generation sequencing (NGS) technologies are a group of automated sequencing techniques used for rapid DNA sequencing. Examples include 454 sequencing, SOLID system, and Illumina Genome Analyzer, which are all designed to sequence millions of short DNA fragments in a single day efficiently. In contrast, the tiled microarray is not a sequencing technology but a technique for gene expression analysis or genotyping which involves hybridization between nucleic acid molecules.
While next-generation sequencers, like 454 sequencing and the Illumina Genome Analyzer, use a synthesis-based approach to sequence DNA, the tiled microarray relies on the binding of DNA fragments to pre-synthesized DNA sequences on a chip to analyze genetic information, which does not equate to sequencing genomes.