Question

Place the statements referring to microRNA biosynthesis in the correct order?

A) Export of pre-microRNA from the nucleus to the cytoplasm, cleavage of pre-microRNA by Dicer, incorporation into the RNA-induced silencing complex (RISC), binding of RISC to target mRNA.
B) Cleavage of pre-microRNA by Dicer, export of pre-microRNA from the nucleus to the cytoplasm, binding of RISC to target mRNA, incorporation into the RNA-induced silencing complex (RISC).
C) Export of pre-microRNA from the nucleus to the cytoplasm, incorporation into the RNA-induced silencing complex (RISC), cleavage of pre-microRNA by Dicer, binding of RISC to target mRNA.
D) Cleavage of pre-microRNA by Dicer, incorporation into the RNA-induced silencing complex (RISC), binding of RISC to target mRNA, export of pre-microRNA from the nucleus to the cytoplasm.

Answer 1

The correct order of microRNA biosynthesis is as follows: cleavage of pre-microRNA by Dicer, incorporation into the RNA-induced silencing complex (RISC), binding of RISC to target mRNA, and export of pre-microRNA from the nucleus to the cytoplasm.

Step-by-step explanation:

1. Cleavage of pre-microRNA by Dicer
2. Incorporation into the RNA-induced silencing complex (RISC)
3. Binding of RISC to target mRNA
4. Export of pre-microRNA from the nucleus to the cytoplasm

Based on the information provided, the correct order of microRNA biosynthesis is as follows:

1. Cleavage of pre-microRNA by Dicer
2. Incorporation into the RNA-induced silencing complex (RISC)
3. Binding of RISC to target mRNA
4. Export of pre-microRNA from the nucleus to the cytoplasm

In this process, pre-microRNA is first cleaved by Dicer, then it is incorporated into the RNA-induced silencing complex (RISC). RISC then binds to the target mRNA, and finally, the pre-microRNA is exported from the nucleus to the cytoplasm.