Final answer:
If a black-coated dog is mated with a brown-coated dog and their litters include both black and brown puppies, it can be concluded that the black-coated dog must be heterozygous for the B allele.
Step-by-step explanation:
In this case, if a black-coated dog (B) is mated with a brown-coated dog (b) and their litters include both black and brown puppies, it can be concluded that the black-coated dog must be heterozygous for the B allele. This means that the black-coated dog has one dominant B allele and one recessive b allele.
When the black-coated dog and brown-coated dog are mated, there is a 50% chance that the black-coated dog passes on the dominant B allele, resulting in black puppies. There is also a 50% chance that the black-coated dog passes on the recessive b allele, resulting in brown puppies.
Therefore, the correct conclusion is that the black dog must be heterozygous for the B allele (option 1).