Final answer:
Rigor in skeletal muscle is caused by a decrease in intracellular Ca²⁺ level and a decrease in ATP level. Additionally, a decrease in ATP level prevents the myosin heads from detaching from the actin-binding sites, further contributing to muscle rigidity.
Step-by-step explanation:
Rigor in skeletal muscle is caused by a decrease in intracellular Ca²⁺ level and a decrease in ATP level.
When the intracellular Ca²⁺ level decreases, the cross-bridges formed between myosin and actin cannot detach, resulting in muscle rigidity.
Additionally, a decrease in ATP level prevents the myosin heads from detaching from the actin-binding sites, further contributing to muscle rigidity.