Question

We can estimate the relative mobility of a population of molecules along the surface of a living cell by fluorescently labeling the molecules of interest, bleaching the label in one small area, and then measuring the speed of signal recovery as molecules migrate back into the bleached area. What is this method called? What does the abbreviation stand for?

1) SDS
2) SPT
3) GFP
4) FRAP

Answer 1

The method described in the question is called Fluorescence Recovery After Photobleaching (FRAP). It is used to estimate the relative mobility of molecules on the surface of a living cell.

Step-by-step explanation:

The method described in the question is called Fluorescence Recovery After Photobleaching (FRAP). FRAP is a technique used to estimate the relative mobility of molecules on the surface of a living cell. In this method, molecules of interest are fluorescently labeled, and a small area of the label is bleached. The speed of signal recovery is then measured as the molecules migrate back into the bleached area.