Question

Write an SOP and batch record for using an individually wrapped, plastic, sterile, disposable 10-mL to dispense exactly 7 mL of solution without contamination.

Answer 1

To dispense exactly 7 mL of solution without contamination, we can follow a standard operating procedure (SOP) and use a batch record. The SOP involves gathering necessary materials, inspecting the pipette, carefully dispensing the solution, and cleaning the work area. The batch record includes recording important information, following the SOP, documenting any deviations, and completing the record with the operator's signature.

Step-by-step explanation:

To dispense exactly 7 mL of solution without contamination, you can follow the standard operating procedure (SOP) and use a batch record. Here's an example of an SOP:

1. Gather all necessary materials, including an individually wrapped, plastic, sterile, disposable 10-mL pipette and a solution to be dispensed.
2. Wash hands thoroughly and put on appropriate personal protective equipment (PPE).
3. Inspect the pipette for any damage or defects, and ensure it is sterile and unopened.
4. Carefully unwrap the pipette, avoiding contact with any surfaces.
5. Hold the pipette vertically and insert the tip into the solution.
6. Squeeze the bulb or plunger to draw the solution into the pipette, ensuring it reaches the 7 mL mark accurately.
7. Remove the pipette from the solution and dispense the solution into the desired container, taking care not to touch the tip to any surfaces that may cause contamination.
8. Dispose of the used pipette appropriately, following proper waste disposal procedures.
9. Clean and sanitize the work area, as well as any equipment used, to prevent any potential contamination.

Here's an example of a batch record:

Date: [Date]

Product: [Solution Name]

Batch Number: [Batch Number]

Operator Name: [Operator Name]

Procedure: [SOP Number]

1. Record all necessary information, including the date, product name, batch number, and operator name.
2. Follow the SOP for accurately dispensing 7 mL of solution using the specified pipette.
3. Double-check the measurement to ensure accuracy.
4. Document any deviations or observations.
5. Complete the batch record with the operator's signature and any additional comments.

Answer 2

To dispense exactly 7 mL of solution without contamination, follow the provided Standard Operating Procedure (SOP) using a sterile, disposable 10-mL pipette.

Step-by-step explanation:

To dispense exactly 7 mL of solution without contamination, you can follow the following Standard Operating Procedure (SOP):

1. Ensure that the individually wrapped, plastic, sterile, disposable 10-mL pipette is unopened and in good condition.
2. Put on sterile gloves and open the sterile packaged pipette.
3. Hold the pipette vertically and use a pipette aid to draw up 7 mL of solution into the pipette.
4. Invert the pipette and let the liquid settle to the bottom to remove any air bubbles. Slowly and carefully release the excess solution back into the original container, leaving exactly 7 mL in the pipette.
5. Place the pipette in a sterile container or directly dispense the solution while taking precautions to avoid touching the tip of the pipette to any non-sterile surfaces.

By following these steps, you can ensure precise and contamination-free dispensing of 7 mL of solution using the individually wrapped, plastic, sterile, disposable 10-mL pipette.