Final answer:
Chloroform is the substance most likely used to disturb the phospholipid bilayer, separate nucleic acids from lipids and proteins, and aggregate nucleic acids.
Step-by-step explanation:
The substance most likely used to disturb the phospholipid bilayer, separate nucleic acids from lipids and proteins, and aggregate nucleic acids is chloroform (option c). Chloroform is a common solvent used in DNA and RNA extraction procedures. It disrupts cell membranes by breaking apart lipid molecules, allowing for the release of nucleic acids.