Final answer:
In Capillary Electrophoresis, the performance is mainly influenced by four factors: voltage, temperature, buffer composition, and sample concentration. These elements impact the speed, resolution, and efficacy of the separation of analytes within the capillary system.
Step-by-step explanation:
The four main aspects that contribute to the limit of performance in Capillary Electrophoresis are voltage, temperature, buffer composition, and sample concentration. These factors affect the efficacy and resolution of the separation process. The applied voltage influences the speed at which the ions move through the capillary. Temperature affects both the viscosity of the buffer solution and the mobility of the analytes. The composition of the buffer determines the pH and ionic strength, which can affect the ionization state of the analytes and their interactions with the capillary wall. Lastly, the sample concentration can influence the separation by causing changes in the conductivity and potential ion-ion interactions in the capillary.