Question

What is the dilution factor when 100 ul is added to a final volume of 5 ml?

a. 1:50
b. 1:55
c. 1:60
d. 1:65

Answer 1

The dilution factor when adding 100 μl to a final volume of 5 mL is 1:51. The answer is found by dividing the total final volume of 5.1 mL by the volume added (0.1 mL).

Step-by-step explanation:

The dilution factor is calculated by dividing the final volume by the initial volume of the solution that is being diluted. In this case, you are adding 100 μl (which is equivalent to 0.1 mL) to a final volume of 5 mL.

To find the dilution factor:

1. Convert 100 μl to mL, which gives 0.1 mL.
2. Add 0.1 mL to the final volume of 5 mL to account for the added volume of the original solution.
3. The total final volume is now 5.1 mL.
4. Calculate the dilution factor by dividing the total final volume by the volume of the solution added: 5.1 mL / 0.1 mL = 51.

Therefore, the dilution factor is 1:51, which is not one of the options provided. It seems there might be a discrepancy in the question or the provided options.