Final answer:
To get your favorite gene into a vector to put somewhere else, you can isolate and amplify the DNA fragments containing the gene. This can be done by screening a genomic library, purifying the DNA, digesting it with restriction enzymes, and using Southern Blotting to identify the fragment with the gene of interest.
Step-by-step explanation:
To get your favorite gene into a vector to put somewhere else, you need to isolate and amplify the DNA fragments containing the gene.
The first step is to screen a genomic library and identify the clone (plaque) that contains the gene of interest. Next, the DNA from this clone is purified, digested with restriction enzymes, and separated by agarose gel electrophoresis. Southern Blotting is then used to transfer and probe the separated DNA fragments.
The smallest DNA fragment containing the gene can be subcloned into a vector for further study.