Question

To follow up on the microarray data you generated on non-melanoma skin cancer tumors, you want to determine whether the Myc transcription factor binds to the promoters of the genes you identified in your microarray. You decide to perform a ChIP assay.

What antibodies will you need to perform this experiment? Make sure to include antibodies for a positive control.

Answer 1

For a ChIP assay to investigate Myc binding to promoters from microarray results in skin cancer, you need an antibody against Myc and a positive control antibody known to bind DNA, like Polymerase II or acetylated histone H3.

Step-by-step explanation:

To determine if the Myc transcription factor binds to the promoters of genes identified in your microarray, you would perform a ChIP assay. For this experiment, you would require a specific antibody against the Myc protein. Additionally, for a positive control, you should use an antibody against a protein that is known to bind to DNA, such as Polymerase II or histone H3, which when acetylated, commonly associates with actively transcribed genes.

It's important to select high-quality and verified antibodies that have a strong reputation for working well in ChIP assays. In the context of cancer research, understanding the interaction between transcription factors like Myc and DNA is crucial since mutations or overexpression can lead to aberrant gene expression and cancer progression.