Final answer:
Methods used to obtain cells for analysis include primary cell culture, karyotyping, and microscopic techniques. Risks associated with these techniques include exposure to carcinogens and unsafe lab practices.
Step-by-step explanation:
Methods used to obtain cells for analysis:
Primary cell culture: Freshly prepared from animal organs or tissues by mechanical scraping or enzymatic method.
Karyotyping: Involves collecting cells from a blood sample or other tissue, stimulating cell division, applying colchicine to arrest chromosomes, and fixing the sample on a slide.
Microscopic techniques: Wet mounts and fixed specimens are used for light microscopy, and various staining and dyeing methods are employed.
Risks associated with these techniques:
Exposure to carcinogens, biohazards, and toxins.
Unsafe lab practices.
Inadequate personal protective equipment.