Question

Which diagnostic tools are used for predicting Diabetic Foot Ulcers (DFUs) based on temperature assessment?

A) X-rays and MRI
B) Echocardiography and Electrocardiography
C) Thermography and Thermometry
D) Blood pressure monitoring and Urinalysis

Answer 1

To detect Clostridium perfringens, an anaerobic environment is essential, utilizing Cooked Meat medium or SPS agar, and incubating at 37°C.

Step-by-step explanation:

To detect Clostridium perfringens (C. perfringens), which is known for causing gas gangrene and can thrive in the necrotic tissue of Diabetic Foot Ulcers (DFUs), specific growth conditions are required. Culturing techniques should aim to replicate the anaerobic environment this bacteria prefers, since C. perfringens is an anaerobe. The use of anaerobic culture methods, including special media like Cooked Meat medium or Sulphite-polymyxin-sulfadiazine (SPS) agar, and culturing in an anaerobic chamber or using anaerobic jars with gas-generating kits can promote the growth of C. perfringens. Incubation at body temperature (37°C), is conducive as it simulates the body's own conditions where this bacterium is typically found.