175k views
1 vote
What are some of the methods of transferring nucleic acids from a gel to a membrane substrate?

1 Answer

2 votes

Final answer:

Nucleic acids are transferred from a gel to a membrane using techniques such as capillary action, vacuum blotting, and electroblotting. Southern and Northern blotting are the respective terms used for the transfer of DNA and RNA to a membrane for subsequent detection by labelled probes.

Step-by-step explanation:

Methods of Nucleic Acid Transfer from Gel to a Membrane

The process of transferring nucleic acids from a gel to a membrane, known as blotting, is essential in molecular biology for detecting specific sequences. The two well-known techniques are Southern blotting for DNA and Northern blotting for RNA.

Once the nucleic acids are immobilized on the membrane, they can be detected using a labelled probe that binds to the target sequence, allowing visualization and identification of specific sequences within the sample.

User Henry Kozurek
by
8.6k points