Question

Shyam took a thin layer of onion peel with a drop of water, then he stained the sample with the solution. After staining, he washed with water and kept it on a clean surface of the slide and with the help of blotting paper, he removed the excess of water. Then he transferred it to a slide and added a drop of glycerine and placed the coverslip on the glass slide to observe the cells under a microscope.

3(a). Coverslip is put on the mounted material on a slide very gently to_________
a. Avoid oozing of stain
b. Avoid oozing of glycerine
c. Avoid entry of air bubbles
d. Avoid the crushing of mounted materials

Answer 1

The coverslip is gently placed over a wet mount to primarily avoid the entry of air bubbles, ensuring clearer microscopic observation of the specimen.

Step-by-step explanation:

When preparing a wet mount in microscopy, the coverslip is gently placed over the specimen to achieve several important objectives. The gentle placement helps to avoid the entry of air bubbles into the specimen, which is critical for clear observation under a microscope. Air bubbles can distort the view, making it difficult to observe the cellular details clearly. While avoiding oozing of stain and glycerine and preventing the crushing of mounted materials are also considerations, the primary reason in this context is to avoid air bubbles, as it has a direct impact on the quality of microscopic observation.