Final answer:
The mixture of Bile Salt and Crystal Violet Dye contains both Gram positive and Gram negative microorganisms. Crystal violet dye stains both types of microorganisms purple, while safranin counterstain helps differentiate between the two types.
Step-by-step explanation:
In the mixture of Bile Salt and Crystal Violet Dye, both Gram positive (+) and Gram negative (-) microorganisms are contained. The crystal violet dye stains both types of microorganisms purple, while the safranin counterstain is used to differentiate between the two types, with Gram positive cells appearing purple and Gram negative cells appearing pink.