Question

Immunoassays can be used to detect viruses or bacteria. Place the steps in the process in the correct order, starting at the top. In this case, a virus is detected.

Step 2

Answer 1

To detect antibodies against bacteria using an EIA, you need to attach antigen from the bacteria to the wells of a microtiter plate. Then add a marker-specific antibody, wash the support, add a second antibody that binds specifically to the primary antibody, and finally add a colorless substrate that is converted to a colored product only if the specific antigen is present. The color intensity can be measured using a spectrophotometer.

Step-by-step explanation:

1. Attach antigen from the bacteria to the wells of a microtiter plate
2. Add marker-specific antibody (primary antibody) to the bound material
3. Wash the support to remove unbound primary antibody
4. Add a second antibody (secondary antibody) that binds specifically to the primary antibody
5. Add a colorless substrate that is converted to a colored product only if the specific antigen is present
6. Measure the color intensity using a spectrophotometer