Final answer:
tRNA adds its amino acid to the polypeptide chain at the A site of the ribosome during protein synthesis.
Step-by-step explanation:
During protein synthesis, transfer RNA (tRNA) adds its amino acid to the growing polypeptide chain at the A site of the ribosome. The ribosome features three sites: A (aminoacyl), P (peptidyl), and E (exit). When a tRNA carrying an amino acid enters into the ribosome, it first comes to the A site, where it is matched with the codon on the mRNA strand. The amino acid is then transferred to the growing polypeptide chain, which is held at the P site. After this transfer, the now uncharged tRNA moves over to the E site before being released back into the cytoplasm.